Coding

Part:BBa_K1114412:Design

Designed by: Shawn Jin   Group: iGEM13_BostonU   (2013-09-07)


DVL0_FB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 241
    Illegal XbaI site found at 268
    Illegal SpeI site found at 1
    Illegal PstI site found at 280
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 241
    Illegal SpeI site found at 1
    Illegal PstI site found at 280
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 241
    Illegal BamHI site found at 262
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 241
    Illegal XbaI site found at 268
    Illegal SpeI site found at 1
    Illegal PstI site found at 280
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 241
    Illegal XbaI site found at 268
    Illegal SpeI site found at 1
    Illegal PstI site found at 280
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 8


Design Notes

This is the MoClo formatted part of lacZ Alpha with FB fusion sites. This is a Level 0 MoClo part with flanking sites F on the 5' side and site B on the 3' side of the part. The fusion site letters refer to 4bp fusion sites: A = GGAG; B = TACT; C = AATG; D = AGGT; E = GCTT; F = CGCT; G = TGCC; H = ACTA. Summary of modifications from original part: Backbone is a modified version of pSB1C3 with added SpeI site in front of gene, BsaI sites flanking, and 4bp fusion sites. See pSB1C3.

Source

igem kit

References